Construction of a protein library of Arabidopsis transcription factors using a wheat cell-free protein production system and its application for DNA binding analysis.
نویسندگان
چکیده
We created a protein library consisting of 647 Arabidopsis transcription factors (TFs) using a wheat cell-free system. The quality of proteins in the library was checked by binding assay of bZIP family proteins. Screening of TFs binding to 5'-regulatory regions of FLC and LFY was conducted using the library, and MYB67 and GBF1 were found to be binding factors.
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عنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 73 7 شماره
صفحات -
تاریخ انتشار 2009